Go to technology
The year before, Nyren’s lab had also spun off a company, Pyrosequencing AB, to refine and commercialize the technology. Pyrosequencing was not the firm that would bring the technology to market, however; that distinction went to Connecticut-based 454 Life Sciences, who licensed whole-genome pyrosequencing applications in 2003. 454 made chips which enabled highly efficient, parallelized sequencing reactions and released the GS20 sequencer in 2005 for the price of $500,000. The GS20 worked by attaching each individual DNA template molecule to a bead and copying it many times using polymerase chain reaction (PCR). Each bead was then loaded into a well in a microplate, where sequencing reactions would be carried out. The light from luciferase activation could be detected through the bottom of the wells, enabling sequences to be read.
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第十条 仲裁实行一裁终局的制度。裁决作出后，当事人就同一纠纷再申请仲裁或者向人民法院提起诉讼的，仲裁机构或者人民法院不予受理。
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（五）法律、法规规定的其他职责。
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